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Cycling parameters for preparation of the library for 16S amplicon sequencing of placental samples
| Step | Temp (°C) | Time | No. of cycles |
|---|---|---|---|
| Initial denaturation | 94 | 3 min | 1 |
| Denaturation | 94 | 30 s | 32 |
| Primer annealing | 60 | 40 s | ↓a |
| Extension | 72 | 90 s | |
| Extension | 72 | 10 min | 1 |
The downward arrow indicates that denaturation, primer annealing, and 90-s extension were carried out over 32 cycles.
