FIG 2.
Expression of solB in recombinant strains. (A) Raw CT values from Q-RT-PCR analysis of solB transcript levels (the average CT value for solB in nontemplate controls was 31.96 ± 0.41). (Note that CT values are inversely proportional to expression levels.) (B) Fold changes in solB overexpression (relative to the WT expression level), derived from Q-RT-PCR analysis. All error bars represent standard deviations (SD) (n = 2). ‡, P ≤ 0.07; *, P ≤ 0.05; **, P ≤ 0.01, ***, P ≤ 0.001, ♢, P ≤ 0.0001 (two-tailed, unpaired t test). (C) Northern blot analysis of solB expression in the WT strain (ATCC 824), the 824(p94_solB) solB overexpression strain (OE), and the 824 ΔsolB strain, using 5 μg total RNA.