TABLE 5.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | ϕ80dlacZΔM15 Δ(lacZY-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) supE44 thi-1 gyrA96 relA1 | Invitrogen |
| E. coli BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| Corallococcus sp. EGB | Wild type, isolated from soil | This study |
| Plasmids | ||
| pMD19-T simple | E. coli cloning vector; Ampr | TaKaRa |
| pET29a (+) | E. coli expression vector, T7 RNA polymerase gene promoter and terminator; Kanr | Novagen |
| pMD19-coMA | pMD19-T simple derivate, containing the CoMA gene from Corallococcus sp. EGB | This study |
| pET29a-coMA | 7.1-kb pET29a derivate carrying the CoMA gene | This study |
| Primers | ||
| F1 | GAATTCCATATGCGCCCCCTCCGCGGACTCTC | |
| R1 | TCCGCTCGAGGCGCAGCCGCCAGATGCC |
a
Underlined sequences within the primers are the NdeI and XhoI restriction sites. Ampr, ampicillin resistance; Kanr, kanamycin resistance.