FIG 1.
Quantitative RT-PCR analysis of gene expression in the wild-type strain of Alternaria alternata. (A) Expression of the thioredoxin peroxidase-coding gene (AaTsa1) in response to H2O2. (B) Expression of the thioredoxin reductase-coding gene (AaTrr1) in response to H2O2. (C) Expression of the glutathione reductase-coding gene (AaGlr1) in response to H2O2. (D) Expression of AaTsa1, AaTrr1, and AaGlr1 in response to tert-butyl-hydroperoxide (t-BHP) or cumene hydroperoxide. The relative expression levels from three independent reactions were calculated using a comparative CT method in relation to the fungal actin gene or comparison of fungal strains grown on medium with or without an oxidant.