The A. alternata thioredoxin reductase 1 gene (AaTrr1), but not the thioredoxin peroxidase 1 gene (AaTsa1), is required for growth and development. (A) Radial growth and formation of hyphae (arrows) and conidia (arrowheads) of the wild-type strain (Z7), two Δtsa1 strains, the CPTsa1 strain expressing a copy of AaTsa1, two Δtrr1 strains, and the CPTrr1 strain expressing a copy of AaTrr1 were cultured on potato dextrose agar or V8 plates for 4 to 7 days. (B) The Δtrr1 strain produces stubby, swelling hyphae, in contrast to those produced by Z7 or other mutants.