FIG 8.

Fluorescence microscopy and differential interference contrast (DIC) imaging of the A. alternata CPTsa1 strain expressing an AaTsa1::GFP::neo fragment and the CPTrr1 strain expressing an AaTrr1::GFP::neo fragment. The AaTsa1 (or AaTrr1) fragment carrying its endogenous promoter was translationally fused in frame with a green fluorescent protein (GFP)-coding gene, followed by fusing with a neo gene cassette under the control of the TrpC promoter and terminator, conferring resistance to G418. Fungal nuclei were stained with 4′-6-diamidine-2-phenylindole (DAPI) fluorescent dye.