FIG 9.
qRT-PCR analyses of the expression of the thioredoxin peroxidase 1-coding gene (AaTsa1) and the thioredoxin reductase 1-coding gene (AaTrr1) in A. alternata strains with impaired redox-responsive transcription regulator YAP1 (Δyap1), Skn7 response regulator (Δskn7), high-osmolarity glycerol 1 (HOG1) MAP kinase (Δhog1), and NADPH oxidases (ΔnoxA, ΔnoxB, ΔnoxR, ΔnoxAB, and ΔnoxAR deletion strains). All strains were treated with 10 mM H2O2 for 30 min after being cultured in thermostatic shakers at 25°C. The relative expression level between the wild-type and the mutant strains was calculated from three independent reactions using a comparative CT method in relation to the actin-coding gene.