TABLE 2.
Oligonucleotide primers used in this studya
| Forward primers |
Reverse primers |
||
|---|---|---|---|
| Primer (gene) | Nucleotide sequence (5′→3′) | Primer (gene) | Nucleotide sequence (5′→3′) |
| 1F (AaTsa1) | GCTTATTGGCGGTACTTAT | 1R (AaTsa1) | TAGGCATTCATTGTTGACCTCCGTTTGGCTGGACCCTTG |
| 2F (AaTsa1) | CAAGGGTCCAGCCAAACGGAGGTCAACAATGAATGCCTA | 2R (AaTsa1) | GCTCCATACAAGCCAAACC |
| 3F (AaTsa1) | CAGCGTCTCCGACCTGA | 3R (AaTsa1) | GAGACAACACGGCTGCGGGATTACCTCTAAACAAGTGTACC |
| 4F (AaTsa1) | GGTACACTTGTTTAGAGGTAATCCCGCAGCCGTGTTGTCTC | 4R (AaTsa1) | TTCCTTTCGTCTTGGGTT |
| 5F (AaTsa1) | GACCGCCGATAGCAACT | 5R (AaTsa1) | CGTAACGCAATACCCTCC |
| 6F (AaTsa1) | GGGGATGGGCATACTTG | 6R (AaTsa1) | CAGATGATAATAATGTCCTCGT |
| 7F (AaTsa1) | TTATGTTTATCGGCACTTTG | 7R (AaTsa1) | TGGACTGGGAGGATTTGA |
| 8F (AaTsa1) | GTTATGCGGATACAGGCG | 8R (AaTsa1) | AGCTCCTCGCCCTTGCTCACCATAAGGATTACCTCTAAACAAGTG |
| 9F (AaTsa1) | CACTTGTTTAGAGGTAATCCTTATGGTGAGCAAGGGCGAGGAGCT | 9R (AaTsa1) | GCCTCTTCGCTATTACGC |
| 10F (AaTrr1) | GAAGGGACCAGCGAAAT | 10R (AaTrr1) | TAGGCATTCATTGTTGACCTCCTGGGTACGGAGAATAAGGA |
| 11F (AaTrr1) | TCCTTATTCTCCGTACCCAGGAGGTCAACAATGAATGCCTA | 11R (AaTrr1) | GCTCCATACAAGCCAAACC |
| 12F (AaTrr1) | CAGCGTCTCCGACCTGA | 12R (AaTrr1) | TTTGTTTGCGACTTCTCAGGGATTACCTCTAAACAAGTGTACC |
| 13F (AaTrr1) | GGTACACTTGTTTAGAGGTAATCCCTGAGAAGTCGCAAACAAA | 13R (AaTrr1) | CTCCACTTACCCACAGCA |
| 14F (AaTrr1) | GCACCGAAATCATCTCCC | 14R (AaTrr1) | CCTGACGATACCGCTTGT |
| 15F (AaTrr1) | TTGGCATAACTGGTGAAGA | 15R (AaTrr1) | GAGGTAAACCCGAAACG |
| 16F (AaTrr1) | GTGGTTTGGCTTGTATGG | 16R (AaTrr1) | TTGGCGAGTGGTGCCTATGT |
| 17F (AaTrr1) | GTTCTGATTTTCGGTAGGC | 17R (AaTrr1) | AGCTCCTCGCCCTTGCTCACCATTAGAAGAGGGTTGGAGCGGTA |
| 18F (AaTrr1) | TACCGCTCCAACCCTCTTCTAATGGTGAGCAAGGGCGAGGAGCT | 18R (AaTrr1) | GCCTCTTCGCTATTACGC |
| 19F (AaTsa1) | AGCAAGAAGGACGGAGGC | 19R (AaTsa1) | GGTGTCGCTTTGATGGTTT |
| 20F (AaTrr1) | GCACCGAAATCATCTCCC | 20R (AaTrr1) | CACCACCTCCGATAACGAC |
| 21F (AaGlr1) | CTGCGTGCCGAAGAAAGT | 22R (AaGlr1) | TCAACCTCGTCCTTAGCG |
| 22F (AaGlr1) | ATCAGCGTCTTCACATACC | 22R (AaGlr1) | TAGGCATTCATTGTTGACCTCCGGACAACGGCAGATACTT |
| 23F (AaGlr1) | AAGTATCTGCCGTTGTCCGGAGGTCAACAATGAATGCCTA | 23R (AaGlr1) | GCTCCATACAAGCCAAACC |
| 24F (AaGlr1) | CAGCGTCTCCGACCTGA | 24R (AaGlr1) | CTCTGGGTGGGCGAAAAGGATTACCTCTAAACAAGTGTACC |
| 25F (AaGlr1) | GGTACACTTGTTTAGAGGTAATCCTTTTCGCCCACCCAGAG | 25R (AaGlr1) | CAGCCAATCAAGCAATAAGG |
| 26F (AaGlr1) | ATTGGTAGTGGAGGTATTGC | 26R (AaGlr1) | GACGGTTGATTCATTCTTTGT |
| 27F (AaGlr1) | GCCACGGAAGGATGAAG | 27R (AaGlr1) | GCTATTTACCCGCAGGAC |
| 28F (AaGlr1) | CTTCCTCCCTTTATTTCAGA | 28R (AaGlr1) | GCCGCACTACAGCACAG |
a
Primer pairings 19F plus 19R (AaTsa1), 20F plus 20R (AaTrr1), and 21F plus 21R (AaGlr1) were used for quantitative RT-PCR. Descriptions of other primers are given in Results and in Materials and Methods.