FIG 1.

Verification of pdeI and pdeR gene functions. (A) AHL production (in Miller units) of recombinant and control E. coli strains after being induced for 3, 6, 9, and 16 h was determined by A. tumefaciens KYC55(pJZ372)(pJZ384)(pJZ410) using a liquid assay. (B) LC-MS chromatograms of C₁₆-HSL from E. coli(pET-pdeI) and the C₁₆-HSL standard. The mass spectra reveal molecular ions [M+H] of m/z 339. (C) Interaction of PdeR with different concentrations of C₁₆-AHL. The PdeR fluorescence was determined at a protein concentration of 34 μM. (D) Titrating PdeR with increasing amounts of C₁₆-AHL. (E) Interaction of PdeR with different concentrations of C₁₀-AHL. (F) Titrating PdeR with increasing amounts of C₁₀-AHL. (G) Interaction of PdeR with different concentrations of pC-HSL. (H) PdeR titration with increasing amounts of pC-HSL.