TABLE 2.

Primers used for construction of QS-defective mutations and promoter-lacZ fusion

Primer name	Primer sequence (5′ to 3′)
pdeI-TYB1-F	AAGGAAAAAAGCGGCCGCCAACGCTACGACGGCTTCAC
pdeI-TYB1-R	GACTAGTTCAAGGAATACATGCCGCAGCGC
pdeI-TYB2-F	AAAACTGCAGGAACGCCGCCGTGTGCATTT
pdeI-TYB2-R	CCGCTCGAGCCCGAAAAGATCGCCGAGAT
pdeR-TYB1-F	AAGGAAAAAAGCGGCCGCTAAGCGATGGTATGGGCCCG
pdeR-TYB1-R	GACTAGTTCATCGAAAAGCCCCAGGCGAT
pdeR-TYB2-F	AAAACTGCAGTTCACGAGATCACGGAGCCG
pdeR-TYB2-R	CCGCTCGAGATAGGAATACATGCCGCAGC
gfp-F	GACTAGTACCAAGAACCCCAGGAGAAGACCATGAGTAAAGGAGAAGAACTTTTCAC
gfp-R	AAAACTGCAGCTATTTGTATAGTTCATCCATGCC
pdeI-F	GGAATTCCCACGGACGACATGGAGT
pdeI-R	GCTCTAGAGCGCGGTGTCGAAAAGGTCT
pdeR-F	GGAATTCGGCGGTTATGTTCGCATCAT
pdeR-R	GCTCTAGAGAAAAGCCCCAGGCGATGGT
hmu-R	GCTCTAGAATCTCGGGGTCCACGGTCAT
hmu-R	GCTCTAGAATCTCGGGGTCCACGGTCAT
fbp-F	GGAATTCGCATCGGGTGCCTGATCGGC
fbp-R	GCTCTAGATCAGCGATCAGCTCGCGCAA