TABLE 3.
Oligonucleotide primers used to validate null mutant strains by diagnostic PCR
| Strain mutant type | Primer | Sequence (5′ to 3′) |
|---|---|---|
| gerPA | gerPA Out For | GTCATGTACATACGGATTTACACCCTAATCAAACGTTCAAAACACACACG |
| gerPA Out Rev | GATAGGGCTTTAATACTTCCGGCAGTACCAATTTGGAACACAGAAGAGG | |
| gerPB | gerPB Out For | CCGGCTCTTTCAACGTTGGAGATAATGTTTCTGTCTACAATTATCA |
| gerPB Out Rev | GCTCTTCTTGGAGTTGGCGCACTTGATCTTCTAAATTTAGGATGGCTGC | |
| gerPC | gerPC Out For | GGTACTGCCGGAAGTATTAAAGCCCTATCTAAATTTTCAAATACGGGCGG |
| gerPC Out Rev | CGGACGACGATACACCGTTCATTTTAATCTGTCCGACTTTTAACTCACGG | |
| gerPD | gerPD Out For | GCATCGACTCCCTTATTATTTATCACAAGCGCAATCATATGAAGGTA |
| gerPD Out Rev | CTCCCCGTATATAACAAGGAATTTCACGGTGGACGGCAATAGCTCTAC | |
| gerPE | gerPE Out For | GCCTTTCTCTCCTTTATTCAGCACATACCGGGAAATTTCCGAAAGGAG |
| gerPE Out Rev | GATCTGCCACATCAGAATCAAATGTATTCGTTGCACTAACACCGTTAAAC | |
| gerPF | gerPF Out For | GCGCACACTTTTAAACTCTGGTGGATTTCAAATTGGAAATGTTGATTATG |
| gerPF Out Rev | TAGTGCCTCTTCACTACTTACTTGTTTATACCACTATTTGTGGAC | |
| gerP | gerP Out For | CGGCGCTAATATTTTCACCTCTGTTAACGGATAATACGCCGCTTCCCCG |
| gerP Out Rev | GATGCTTGCAGCAGAAGAAGCATATCAAGATTGGCAAGGGAAGAGTG |