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SDS-PAGE analysis of E. coli expression and copurification of KshAN and KshBN. (a) KshA1N and KshBN. (b) KshA2N and KshBN. Lane 1, standard marker protein; 2, cell extracts from BL21-kshAN; 3, cell extracts from BL21-kshBN; 4, mixture of cell extracts of BL21-kshAN and BL21-kshBN in a KshAN:kshBN molar ratio of approximately 1:2; 5, the copurified fraction of KshAN and KshBN with 150 mM imidazole.
