TABLE 4.
Profiles of phytosterol transformation by 9-OHAD-producing strains for 120 h
| Strain and concn of substratea (g liter−1) | Major product | Concnb (g liter−1) | Molar yieldc (%) | Yield ratio of 9-OHAD (%) |
|---|---|---|---|---|
| IV | ||||
| 2 | 9-OHAD | 1.10 ± 0.05 | 74.8 ± 3.1 | 95.7 ± 0.2 |
| AD | 0.05 ± 0.02 | 3.4 ± 1.0 | ||
| 20 | 9-OHAD | 10.04 ± 0.22 | 68.3 ± 1.5 | 89.0 ± 0.5 |
| AD | 1.24 ± 0.11 | 8.4 ± 0.5 | ||
| V | ||||
| 2 | 9-OHAD | 1.32 ± 0.03 | 90.2 ± 1.9 | 97.6 ± 0.3 |
| AD | 0.03 ± 0.01 | 2.2 ± 0.3 | ||
| 20 | 9-OHAD | 11.71 ± 0.15 | 79.8 ± 1.0 | 94.2 ± 0.4 |
| AD | 0.71 ± 0.10 | 4.9 ± 0.6 |
a
The conversion of 2 g liter−1 phytosterols was performed in shake flasks using growing cells, and the conversion of 20 g liter−1 phytosterols was performed in a 7-liter bioreactor using resting cells. Data are shown as the averages from triplicate experiments.
b
Amounts of major metabolites and their molar yields were determined from three independent transformations and are expressed as the actual range from the least to the topmost.
c
The yield ratio of 9-OHAD was obtained by calculating the molar yield of 9-OHAD among all the products.