TABLE 1.
Strains used in this study
| Strain | Genotype, description, or relevant characteristics | Source |
|---|---|---|
| Escherichia coli | ||
| Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Nalr) endA1 nupG | Invitrogen |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild-type P. aeruginosa strain | J. D. Shrout |
| PAOYs1 | pslBCD inactivated in PAO1; nonresistant | Present study |
| PAOYs2 | pelB inactivated in PAO1; nonresistant | J. D. Shrout |
| PAOYs3 | algD inactivated in PAO1; nonresistant | J. D. Shrout |
| PAOYs4 | pilA inactivated in PAO1; nonresistant | Present study |
| PAOYs5 | cdrAB inactivated in PAO1; nonresistant | Present study |
| PAOYs6 | algU inactivated in PAO1; nonresistant | Present study |
| PAOYs7 | rpoS inactivated in PAO1; nonresistant | Present study |
| PAOYs8 | ppyR inactivated in PAO1; nonresistant | Present study |
| PAOYs9 | lasR inactivated in PAO1; nonresistant | J. D. Shrout |
| PAOYs10 | amrZ inactivated in PAO1; nonresistant | Present study |
| PAOYs11 | rsmA inactivated in PAO1; nonresistant | Present study |
| PAOYs12 | rsmA and pslBCD inactivated in PAO1; nonresistant | Present study |
| B0034psl-PAO1 | PAO1 in which the RBS region of psl operon was replaced with a constitutive prokaryotic RBS BBa_B0034 (iGEM biobrick); nonresistant | Present study |
| PAO1::RYS-1 | PAO1 containing a gene encoding EGFP directly under the control of the psl promoter integrated at the attTn7 site; Gmr | Present study |
| PAO1::RYS-2 | Strain for amplifying signal. PAO1 integrated by the inverted Psl expression reporter RYS-2 at the attTn7 site; Gmr | Present study |
| pslBCD::RYS-2 | ΔpslBCD integrated by RYS-2 at the attTn7 site; Gmr | Present study |
| B0034psl::RYS-3 | ΔpslBCD integrated by RYS-3 at the attTn7 site; Gmr | Present study |
| PAO1::RYS-1a | Gene marker of Gmr of PAO1::RYS-1 is excised; nonresistant | Present study |
| PAO1::RYS-2a | Gene marker of Gmr of PAO1::RYS-2 is excised; nonresistant | Present study |
| pslBCD::RYS-2a | Gene marker of Gmr of pslBCD::RYS-2 is excised; nonresistant | Present study |
| B0034psl::RYS-3a | Gene marker of Gmr of B0034psl::RYS-3 is excised; nonresistant | Present study |
| PAO1::RYS-4 | PAO1 harboring the RsmY reporter, RYS-4; Gmr | Present study |
| PAO1::RYS-5 | PAO1 harboring the RsmZ reporter, RYS-5; Gmr | Present study |
| PAO1::RYS-6 | PAO1 harboring the RsmA reporter, RYS-6; Gmr | Present study |
| PAO1-egfp | PAO1 tagged by EGFP integrated at the attTn7 site; Gmr | Present study |
| B0034psl-egfp | B0034psl-PAO1 tagged by EGFP at the attTn7 site; Gmr | Present study |
| pslBCD-egfp | ΔpslBCD tagged by EGFP at the attTn7 site; Gmr | Present study |