FIG 6.

Addition of the *ASV SsrA tag to GFP increases the turnover rate in V. fischeri. ES114 cells carrying pJLS150 (GFP*ASV) (squares) or pJLS153 (parental GFP with no SsrA tag) (circles) were grown in SWTO medium plus 2 mM IPTG or SWTO medium with no inducer to an OD₅₉₅ of 1.5 before being washed and resuspended in SWTO medium without IPTG. GFP/OD₅₉₅ values were taken every 15 min for 4 h. Values under noninduced conditions (no IPTG added) were subtracted as the background from values under induced conditions. Error bars indicating standard deviations (n = 3) are smaller than the symbols. Data from one representative experiment of four performed are shown.