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. 2018 Jul 3;49:55. doi: 10.1186/s13567-018-0551-9

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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CRCoV co-localize with caveolin. Cells treated with virus were synchronized on ice for 60 min and incubated at 37 °C for 20, 40, 60, or 120 min before they were washed and fixed. Caveolin-1 are presented on A (red channel) while B shows stained virus nucleocapsid protein (green channel). C Depicts merged image with marked co-localization. D FITC conjugated CHTxB (green) co-localizing with caveolin-1 (red) after 40 (upper) and 60 (lower) min. E Negative controls for staining. Scale bar 10 µm.