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. 2018 May 23;4(2):95–103. doi: 10.1159/000488984

Fig. 1.

Fig. 1.

Wnt3a stimulates macrophage proliferation. a, b Cell counting (a) and MTT assay (b) in Raw 264.7 cells. * p < 0.05 versus cells at day 0, n = 5; #p < 0.05 versus cells without Wnt3a treatment at the respective time, n = 5. c, d Cell counting (c) and MTT assay (d) in BMMs. * p < 0.05 versus cells at day 0, n = 5; #p < 0.05 versus cells without Wnt3a treatment at the respective time, n = 5. e Representative immunofluorescent staining images (left) and quantitative analysis (right) for Ki67 in Raw 264.7 cells. * p < 0.05 versus cells treated with vehicle alone, n = 4. Cells were stained with DAPI to visualize the nuclei. f Representative immunofluorescent images (left) and quantitative analysis (right) for Ki67 in BMMs. * p < 0.05 versus cells treated with vehicle alone, n = 4; #p < 0.05 versus cells stimulated with M-CSF for 24 h, n = 4. BMMs, bone marrow-derived macrophages; DAPI, 4′,6-diamidino-2-phenylindole; M-CSF, macrophage colony-stimulating factor; MTT, 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide.