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. 2018 Jul 3;11:428. doi: 10.1186/s13104-018-3537-9

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — HI titers in serum against H9N2 AIV. Average serum HI titers 21 and 28 days ph, from 10 chickens per group. Chickens were vaccinated in ovo on ED18 and received a second vaccination 14 days ph. Vaccines consisted of 15 µg of BPL inactivated H9N2 virus administered alone (BPL), with AddaVax™ (BPL + Add), or with 2 µg CpG ODN 2007 (BPL + CpG). One group received just PBS as a negative control (PBS). Serum was collected weekly following hatch. HI titers were first observed in serum 7 days post secondary vaccination. Group means that share the same letter did not differ significantly. Standard error of the mean is indicated with error bars. Data were analyzed using Duncan’s Multiple Range Test and differences in means were considered significant if p < 0.05