Fig. 2.

Upregulation of IL6 stimulated by E2 in NSCLC cell lines, A549 and H975. (a) ERβ/IL6 expression was up regulated in NSCLC cell lines compared with normal pneumocytes (HBE). (b) Synchronized cells were treated with E2 at different concentrations (0 nM, 1 nM, 10 nM and 100 nM) and Ful (0.1 μM and 1 μM) for 2 days. The protein expression of ERβ and IL6 was analyzed using western blot. (c) Synchronized cells were treated with E2 at different time points (0 h, 6 h, 12 h, 24 h, 48 h and 72 h) at concentrations of 10 nM. The protein expression of ERβ and IL6 was analyzed using western blot. GAPDH was used as a control. (d) Autocrine IL6 was analyzed by ELISA assay after concentration-dependent treatment of E2 (10 nM) or Ful (0.1uM). (e) The upregulation of IL6 in A549 after stimulation with E2 (10 nM) or Ful (0.1uM) was determined by immunofluorescence