Fig. 1.

Co-localization of H4K20me3 with H3K4me3 and RNAPII in ES cells. a Venn diagrams showing overlap between H4K20me3 and H3K4me3, H4K20me3 and RNAPII, H4K20me3/H3K4me3/RNAPII, H4K20me3 and H3K9me3, and H3K4me3/RNAPII/H4K20me3/H3K9me3 co-occupied regions. b Annotation of H4K20me3 and H3K4me3, and H3K9me3 and H3K4me3 co-occupied regions using HOMER software [40]. Scatter plots of (c) H4K20me3 and H3K4me3 densities, d H4K20me3 and RNAPII, e H4K20me3 and H3K9me3 densities, and (f) H3K9me3 and H3K4me3 densities (RPKM) at 2 kb genomic bin intervals. g Density of H3K4me3 and H4K20me3 at H3K4me3 peaks (left panel), H4K20me3 peaks (middle panel), H4K20me3/H3K4me3 intersecting regions, H3K4me3 only peaks, or H4K20me3 only peaks (right panel). h UCSC browser view of H4K20me3, H3K9me3, H3K4me3, and RNAPII co-occupancy in ES cells. i Heat maps of H3K4me3, RNAPII, H4K20me3, and H3K9me3 densities at H4K20me3/H3K4me3 marked regions. Rows were sorted by the level of H4K20me3 at H4K20me3/H3K4me3 regions. j Distribution of H4K20me3, H3K9me3, H3K4me3 and H4K20me3/H3K4me3 co-occupied ChIP-Seq peaks in ES cells. k Enriched DNA binding motifs from Chen et al. [41] in H4K20me3/H3K4me3, H3K4me3-only, and H4K20me3-only co-occupied regions identified using MEME-ChIP software [42]. The percent abundance of motifs is shown below the sequence logo