Fig. 3.

p53R248Q and p53R273C mutants but not p53 wild-type transactivate the HER2 promoter in stably transfected Saos-2 cells. Transcriptional activity of the HER2 promoter was assessed by a Luciferase reporter assay in Saos-2 cells stably transfected with R248Q and R273C p53 mutants, as well as with wild-type p53 plasmid and empty vector. Stably transfected Saos-2 cells were co-transfected with pNeuLite luciferase reporter plasmid for the HER2 promoter. After 48 h, luciferase activity was measured as described in the Materials and Methods. The results of the luciferase activity were normalized to renilla activity and are reported as the mean ± SEM of three independent experiments. Statistical analysis was performed with t-student test by comparing the results obtained for the Relative Activity in Saos-2 cells transfected with p53R48Q or p53R273C or wtp53 against empty vector: * p < 0.05