. 2018 Jun 19;2018:7807245. doi: 10.1155/2018/7807245

Table 1.

Acute effects of exercise on circulating extracellular vesicles.

Author	Exercise dose	Subtype EV	Preanalytical phase	Collection time	Response
Harrison et al. [76]	Cycling for 90 min at 70% VO₂peak followed by ten 1 min sprints interspersed with 1 min of recovery in young, recreationally active men.	CD31+/CD42b-	1600 g for 15 min at 4°C	Morning following exercise bout	No change

Mobius-Winkler et al. [63]	4 hr cycling at 70% IAT in young, healthy men.	CD42b⁻CD42b⁻/CD62E⁺	11,000g for 2 min	16 predefined time points during and after finishing cycling.	No change

Jenkins et al. [74]	Exercise at 70% of VO₂peak until 598 kcal was expended in recreationally, healthy active men.	CD31⁺/CD42b⁻CD62E⁺	Double centrifugation of 1500g for 20 min at room temperature	(i) Pre-meal (ii) 1, 2, 3, and 4 hr postprandial	↓CD31⁺/CD42b⁻ ↓CD62E⁺ Unaffected by high-fat meal

Guiraud et al. [60]	Single session of HIIE: 15-second intervals at 100% of PPO and 15-second passive recovery intervals or isocaloric MICE in men with coronary heart disease.	CD31⁺ CD62E⁺ CD42b⁻ CD42b⁺	1500g for 15 min followed by a single centrifugation at 13,000g for 2 min	(i) 10 min pre-ex (ii) 20 min post-ex (iii) 24 hr post-ex (iv) 72 hr post-ex	No change

Ross et al. [62]	3 sets of 6 resistance exercises at 15 RM w/o rest in young, trained men.	CD144⁺ CD146⁺ CD105⁺	1500g for 15 min followed by 13,000g for 2 min	(i) Pre-ex (ii) 10 min post-ex (iii) 2 hr post-ex (iv) 24 hr post-ex	No change

Wahl et al. [67]	(1) HVT; 130 min at 55% PPO, (2) 4 × 4 min at 95% PPO, and (3) 4 × 30 sec all-out in healthy male, triathletes.	CD31⁺/CD42b⁻ CD31/CD42b CD14/CD16	1.861g for 10 min at 4°C	(i) Pre-ex (ii) Immediately post-ex (iii) 60 min post-ex (iv) 180 min post-ex	↓CD31⁺/CD42b⁻ ↓CD31/CD42b ↓CD14/CD16

Chanda et al. [61]	Acute strenuous exercise (treadmill running VO₂peak) versus. moderate (75% HR_max for 45 min) in healthy females.	Total EVs CD41a	2500 rpm for 5 min at 4°C	(i) Pre-ex (ii) Immediately post-ex (iii) 45 min post-ex	↑Total EVs ↑CD41a Only after strenuous ex; No moderate ex change

Durrer et al. [72]	(1) HICE 20 min cycling at just above VT. (2) HIIE 10 × 1 min at 90% peak aerobic power in young, overweight, inactive males and females.	CD62E⁺ CD31⁺/CD42b⁻	Double centrifugation of 1500g for 15 min at room temperature	(i) Pre-ex (ii) Immediate post-ex (iii) Morning post-ex	↑CD62E⁺ (females only) No change CD31⁺/CD42b⁻ ↓CD62E⁺ (males only) ↓CD31⁺/CD42b⁻

Lansford et al. [69]	Acute bout at 60–75% VO₂peak in healthy, active individuals	CD62E⁺, CD34⁺ CD31⁺/CD42b⁻	Double centrifugation of 1500g for 20 min at room temperature	(i) Pre-ex (ii) Post-ex	↑CD62E⁺ in men ↑CD34⁺ in women No change CD31⁺/CD42b⁻

Wilhelm et al. [65]	1 h of moderate- (46 ± 2% VO₂peak) or heavy- (67 ± 2% VO₂peak) intensity semirecumbent cycling in healthy, young men.	CD62E⁺ CD41⁺	17,500g for 1 hr at 4°C	(i) Pre-ex (ii) Post-ex	↑CD41⁺ following heavy exercise No CD62E⁺ change

Bei et al. [114]	Exercise stress test in middle-aged, overweight men and women.	CD63⁺	1000g for 10 min and 2500g for 15 min at room temperature	(i) Rest (ii) Peak-ex (iii) Recovery (15 min after completion	↑EV count

Whitham et al. [66]	1 hr (30 min at 55%, 20 min at 70%, and ~10 min at 80% of VO₂peak) in healthy males.	ACTN4, ADAM10, ALIX, ANAX11, and CD81	20,000g for 2x at 60 min	(i) Pre-ex (ii) Exercise (iii) 4 hr post-ex	↑EV count

IAT: individual anaerobic threshold; HIIE: high-intensity interval exercise; MICE: moderate-intensity continuous exercise; RM: repetition max; HVT: high-volume training; PPO: peak power output; HICE: high-intensity continuous exercise; VT: ventilatory threshold.