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. 2018 Apr 19;103(7):1235–1244. doi: 10.3324/haematol.2017.185637

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Figure 1. — Munc13 expression and deletion. (A) RT-qPCR of all Munc13 proteins relative to β-actin in C57BL/6J platelets. N=3. (B) RT-qPCR from Munc13-2^−/− platelets. All values are relative to β-actin and Munc13 isoform levels in Munc13-2^+/+ platelets (WT). N=3. Bar: mean; error bar: Standard Error of Mean. (C) Representative immunoblots of platelet and spleen lysates from all Munc13-4 mutant mice probed with anti-mouse Munc13-4 antibody. β-actin was used as loading control. Numbers below blots represent densitometry results relative to β-actin and Munc13-4^+/+. ND: not detected.