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. 2018 Jun 29;13:3805–3825. doi: 10.2147/IJN.S162836

Figure 2.

Figure 2

Confocal laser-scanning microscopy of PEI-EV (stained green with WGA Alexa Fluor 488) and hPDLSC (gray) interaction using different PEI concentrations. Notes: hPDLSCs incubated with EVs coated with PEI at 5 mg/mL (A1–A3); 0.5 mg/mL (B1–B3); 0.1 mg/mL (C1–C3), 0.05 mg/mL (D1–D3), and 0.025 mg/mL (E1–E3). PEI-EVs stained in green with WGA Alexa Fluor 488 (A1–E1). Light-transmission channels showed cell morphology (gray; A2–E2); merged images of the aforementioned channels (A3–E3). (F) Trypan blue exclusion test to evaluate the effects of different PEI concentrations on hPDLSC viability. 0.05 mg/mL PEI concentration was chosen for the other experiments. **P<0.01 compared to the other PEI concentrations; n=3; scale bars =10 μm.

Abbreviations: PEI, polyethylenimine; EV, extracellular vesicle; WGA, wheat-germ agglutinin; hPDLSC, human periodontal-ligament stem cell.