FIG. 4.

Time course analyses on the effects of HC on Glut2 and Gck expression. Day-18 cells from (A) MIP-EGFP or (B) R1 mES cells received 1 or 10 μM HC or DMSO control for 3, 6, or 9 days. The resulting cells were analyzed by qRT-PCR using β-Actin as the internal control. Six days of HC treatment, compared to the DMSO control, were sufficient to induce higher expression of Glut2 and Gck in both ES cell lines. Therefore, in some subsequent experiments we used an endpoint of 6 days post-HC incubation. Data represent mean ± SD of three independent experiments. Significance was determined by one-way ANOVA. * indicates P < 0.05 compared to the DMSO control.