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. 2018 Jul 1;27(13):898–909. doi: 10.1089/scd.2017.0160

FIG. 7.

FIG. 7.

Islets from mice with beta cell-specific knockout of GR have reduced gene expression of Glut2 and do not exhibit glucose-stimulated insulin secretion in vitro. P8 islets were isolated from mice that had beta cell-specific knockout of GR (GR β-cell-KO) or from control (Con) littermates. (A) Gene expression was analyzed by qRT-PCR using β-Actin as an internal control and expressed as fold change compared to the control mice. Data represent mean ± SD from six independent experiments. Significance was determined by t-test. *** indicates P < 0.001 compared to control. n.s. indicates not significant. (B) P8 islets were sequentially stimulated with low (0.5 mM) followed by high (17.0 mM) concentrations of d-glucose. C-PEPTIDE concentrations in the media were determined by enzyme linked immunosorbent assay. Stimulation index is defined as the C-PEPTIDE concentration in high glucose divided by C-PEPTIDE concentration in low glucose. Data represent mean ± standard error of the mean from three independent experiments. Significance was determined by t-test. * Indicates P < 0.05.