Fig 2. Effect of AKBA on induction of apoptosis in A172 cells.

(A) A172 cells were treated with AKBA for 72 hrs and apoptosis was assessed using Annexin-V-FITC/PI double staining and flow cytometry. Double negative cells indicated the live cell population, Annexin-V-FITC positive cells indicated the early apoptotic population, double positive cells indicated late apoptotic population and PI positive cells indicated the necrotic population. (B) Cells were treated with 30 μM AKBA for 24 and 48 hrs. Cell lysates from A172 monolayers were subjected to Western blot analysis with mouse anti-human PARP and β-actin antibodies.