Fig 3. Overexpression of Slug and Snail in SAS cells.

(A, B, and C) SAS cells transfected with plasmids encoding either HA-tagged Slug or Snail were subjected to immunoblot (A) and qRT-PCR analyses (B and C). α-tubulin was used as a loading control (A). mRNA levels were normalized to the amount of GAPDH mRNA (B and C). (D and E) SAS cells transfected with plasmids encoding either HA-tagged Slug or Snail were exposed to docetaxel (DTX; 10 μM) (D) or Erlotinib (5 μM) (E) for 24 h. The viable cells were trypsinized and counted using a hemocytometer. The value of the control cells is indicated as “1”. (F) Invasion assays were performed on SAS cells transfected with either HA-tagged Slug or Snail. The value of the control cells is indicated as “1”. Each value represents the mean ± s.d. of triplicate determinations from a representative experiment. Similar results were obtained in at least three independent experiments. Cont., negative control plasmid. p values were determined by Student’s t-test. **p < 0.01.