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. 2018 Jul 3;13(7):e0199780. doi: 10.1371/journal.pone.0199780

Table 1. Details of the samples, genetic diversity and haplotype composition of the B. clarkeana populations surveyed, based on cpDNA (psbA-trnH, rps12-rpl20, trnL-trnF), ITS and EST-SSR sequences.

Code Locationa Longitude (°E) Latitude
(°N)
Size EST-SSRb cpDNA ITS
NA PIC HO HE FIS Haplotype h (±SD) π×103
(±SD×103)
Haplotype h (±SD) π×103
(±SD×103)
ZSC T 120°79’ 29°40’ 21 1.533 0.1 0.093 0.118 0.373 C1,C2 0.15(±0.13) 0.08(±0.07) R1,R2 0.15(±0.13) 0.24(±0.19)
ZTC T 119°44’ 30°32’ 18 1.267 0.076 0.022 0.096 0.779 C1 0 0 R3 0 0
ZLQ T 118°90’ 30°12’ 24 1.267 0.067 0.056 0.082 0.338 C2 0 0 R2 0 0
ANS H 118°88’ 30°68’ 22 1.667 0.085 0.025 0.093 0.744 C2 0 0 R3 0 0
ANY H 118°13’ 30°89’ 24 1.333 0.092 0.125 0.117 -0.051 C2 0 0 R1,R2,R4 0.39(±0.15) 0.78(±0.33)
ACT H 117°63’ 30°34’ 24 1.533 0.095 0.111 0.111 0.017 C2 0 0 R1 0 0
AQL H 117°49’ 29°88’ 24 1.4 0.088 0.106 0.105 0.012 C2 0 0 R1 0 0
JGD G 114°56’ 28°54’ 24 1.133 0.008 0.008 0.008 -0.015 C3 0 0 R5 0 0
HSP S 110°85’ 31°75’ 20 1.267 0.079 0.074 0.09 -0.043 C4 0 0 R6,R7,R8 0.67(±0.08) 1.51(±0.20)
HXS S 110°64’ 31°34’ 14 1.007 0.005 0.005 0.005 0 C4 0 0 R8 0 0
HZS W 110°40’ 30°84’ 24 1.4 0.068 0.036 0.082 0.607 C4 0 0 R8 0 0
HZL Z 110°45’ 29°09’ 24 1.333 0.079 0.089 0.096 0.101 C5 0 0 R9,R10 0.57(±0.12) 3.54(±0.74)
HYJ Z 110°44’ 28°78’ 24 2.333 0.27 0.278 0.309 0.121 C5 0 0 R11 0 0
CNJ N 107°20’ 29°10’ 24 1.333 0.037 0.014 0.043 0.688 C6 0 0 R12 0 0
SNX D 106°93’ 32°44’ 24 1.13 0.024 0.023 0.028 0.193 C7,C8 0.41(±0.13) 0.22(±0.13) R13,R14 0.53(±0.08) 1.64(±0.24)
SWM D 106°54’ 32°57’ 20 1.067 0.003 0.003 0.003 0 C7 0 0 R13 0 0
SLB Q 106°08’ 33°58’ 24 1.133 0.023 0.029 0.027 -0.048 C7 0 0 R13 0 0
SLG Q 105°94’ 33°30’ 15 1 0 0 0 NA C7 0 0 R13 0 0
AVERAGE 1.34 0.07 0.06 0.08 0.22 0.03 0.17 0.16 0.43
TOTAL 394 8 0.82(±0.01) 3.12(±0.13) 14 0.88(±0.01) 6.39(±0.30)

Notes

a: H, Huang Mts.; T, Tianmu Mts.; G, Guan Mts.; W, Wu Mts.; S, Shennongjia Mts.; Z, Zhangjiajie Mts.; N, Nan Mts.; Q, Qinling Mts.; D, Daba Mts.

b, NA, number of alleles per locus across all populations; PIC, polymorphic information content; HO, observed heterozygosity (mean value); HE, expected heterozygosity (mean value); FIS, inbreeding coefficient; h haplotype diversity; π, nucleotide diversity; SD, standard deviation; NA, not available.