Table 1. Details of the samples, genetic diversity and haplotype composition of the B. clarkeana populations surveyed, based on cpDNA (psbA-trnH, rps12-rpl20, trnL-trnF), ITS and EST-SSR sequences.
Code | Locationa | Longitude (°E) | Latitude (°N) |
Size | EST-SSRb | cpDNA | ITS | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NA | PIC | HO | HE | FIS | Haplotype | h (±SD) | π×103 (±SD×103) |
Haplotype | h (±SD) | π×103 (±SD×103) |
|||||
ZSC | T | 120°79’ | 29°40’ | 21 | 1.533 | 0.1 | 0.093 | 0.118 | 0.373 | C1,C2 | 0.15(±0.13) | 0.08(±0.07) | R1,R2 | 0.15(±0.13) | 0.24(±0.19) |
ZTC | T | 119°44’ | 30°32’ | 18 | 1.267 | 0.076 | 0.022 | 0.096 | 0.779 | C1 | 0 | 0 | R3 | 0 | 0 |
ZLQ | T | 118°90’ | 30°12’ | 24 | 1.267 | 0.067 | 0.056 | 0.082 | 0.338 | C2 | 0 | 0 | R2 | 0 | 0 |
ANS | H | 118°88’ | 30°68’ | 22 | 1.667 | 0.085 | 0.025 | 0.093 | 0.744 | C2 | 0 | 0 | R3 | 0 | 0 |
ANY | H | 118°13’ | 30°89’ | 24 | 1.333 | 0.092 | 0.125 | 0.117 | -0.051 | C2 | 0 | 0 | R1,R2,R4 | 0.39(±0.15) | 0.78(±0.33) |
ACT | H | 117°63’ | 30°34’ | 24 | 1.533 | 0.095 | 0.111 | 0.111 | 0.017 | C2 | 0 | 0 | R1 | 0 | 0 |
AQL | H | 117°49’ | 29°88’ | 24 | 1.4 | 0.088 | 0.106 | 0.105 | 0.012 | C2 | 0 | 0 | R1 | 0 | 0 |
JGD | G | 114°56’ | 28°54’ | 24 | 1.133 | 0.008 | 0.008 | 0.008 | -0.015 | C3 | 0 | 0 | R5 | 0 | 0 |
HSP | S | 110°85’ | 31°75’ | 20 | 1.267 | 0.079 | 0.074 | 0.09 | -0.043 | C4 | 0 | 0 | R6,R7,R8 | 0.67(±0.08) | 1.51(±0.20) |
HXS | S | 110°64’ | 31°34’ | 14 | 1.007 | 0.005 | 0.005 | 0.005 | 0 | C4 | 0 | 0 | R8 | 0 | 0 |
HZS | W | 110°40’ | 30°84’ | 24 | 1.4 | 0.068 | 0.036 | 0.082 | 0.607 | C4 | 0 | 0 | R8 | 0 | 0 |
HZL | Z | 110°45’ | 29°09’ | 24 | 1.333 | 0.079 | 0.089 | 0.096 | 0.101 | C5 | 0 | 0 | R9,R10 | 0.57(±0.12) | 3.54(±0.74) |
HYJ | Z | 110°44’ | 28°78’ | 24 | 2.333 | 0.27 | 0.278 | 0.309 | 0.121 | C5 | 0 | 0 | R11 | 0 | 0 |
CNJ | N | 107°20’ | 29°10’ | 24 | 1.333 | 0.037 | 0.014 | 0.043 | 0.688 | C6 | 0 | 0 | R12 | 0 | 0 |
SNX | D | 106°93’ | 32°44’ | 24 | 1.13 | 0.024 | 0.023 | 0.028 | 0.193 | C7,C8 | 0.41(±0.13) | 0.22(±0.13) | R13,R14 | 0.53(±0.08) | 1.64(±0.24) |
SWM | D | 106°54’ | 32°57’ | 20 | 1.067 | 0.003 | 0.003 | 0.003 | 0 | C7 | 0 | 0 | R13 | 0 | 0 |
SLB | Q | 106°08’ | 33°58’ | 24 | 1.133 | 0.023 | 0.029 | 0.027 | -0.048 | C7 | 0 | 0 | R13 | 0 | 0 |
SLG | Q | 105°94’ | 33°30’ | 15 | 1 | 0 | 0 | 0 | NA | C7 | 0 | 0 | R13 | 0 | 0 |
AVERAGE | 1.34 | 0.07 | 0.06 | 0.08 | 0.22 | 0.03 | 0.17 | 0.16 | 0.43 | ||||||
TOTAL | 394 | 8 | 0.82(±0.01) | 3.12(±0.13) | 14 | 0.88(±0.01) | 6.39(±0.30) |
Notes
a: H, Huang Mts.; T, Tianmu Mts.; G, Guan Mts.; W, Wu Mts.; S, Shennongjia Mts.; Z, Zhangjiajie Mts.; N, Nan Mts.; Q, Qinling Mts.; D, Daba Mts.
b, NA, number of alleles per locus across all populations; PIC, polymorphic information content; HO, observed heterozygosity (mean value); HE, expected heterozygosity (mean value); FIS, inbreeding coefficient; h haplotype diversity; π, nucleotide diversity; SD, standard deviation; NA, not available.