Fig 2. Copy numbers of candidate miRNAs determined by Illumina deep sequencing and validation of their expression levels using stem-loop qRT-PCR.

A: Reads of the six selected miRNAs obtained from PRRSV-infected MARC-145 cells are shown along the Y-axis (black bars). B: MARC-145 cells were infected with vAPRRS at an MOI of one, and miRNA expression was determined at 24 hpi using stem-loop qRT-PCR. Relative expression levels were normalized against snRNA U6. Data are shown as the mean ± standard deviation (SD) of three independent experiments.