. 2018 Jul 3;13(7):e0200029. doi: 10.1371/journal.pone.0200029

Table 2. Primers used to determine the relative expression level of host miRNAs by stem-loop qRT-qPCR and viral ORF7 RNA.

Primer	Sequence (5′−3′)
RT-miR-21	`TGTCGGGTAGCTTATCAGACTGATGTTGACTGTTGAATCTCATGGCAACACCAGTCGATGGGCTGTCTGACAAACATCAG`
RT-miR-140	`UGUGUCUCUCUCUGUGUCCUGCCAGUGGUUUUACCCUAUGGUAGGUUACGUCAUGCUGUUCUACCACAGGGUAGAACCACGGACAGGAUACCGGGGCACCCCGTGGTT`
RT-miR-185	`AGGGCGCGAGGGATTGGAGAGAAAGGCAGTTCCTGATGGTCCCCTCCTCAGGGGCTGGCTTTCCTCTGGTCCTTCCCTCCCATCAGGAAC`
RT-miR-26a	`GTGGCCTCGTTCAAGTAATCCAGGATAGGCTGTGCAGGTCCCAATGGGCCTATTCTTGGTTACTTGCACGGGGACGCAGCCTATC`
RT-miR-505	`GATGCACCCAGTGGGGGAGCCAGGAAGTATTGATGTTTCTGCCAGTTTAGCGTCAACACTTGCTGGTTTCCTCTCTGGAGCATCAGGAAACC`
RT-miR-199a	`AGGAAGCUUCUGGAGAUCCUGCUCCGUCGCCCCAGUGUUCAGACUACCUGUUCAGGACAAUGCCGUUGUACAGUAGUCUGCACAUUGGUUAGACUGGGCAAGGGAGAGCAGAACAGGT`
Q-miR-21(F)	`TAGCTTATCAGACTGATGTTGAT`
Q- miR-140 (F)	`GGGGTACCACAGGGTAGAAC`
Q-miR-185(F)	`GGTGGAGAGAAAGGCAGTTCCTGA`
Q-miR-26a (F)	`TTCAAGTAATCCAGGATAGGCTG`
Q-miR-505 (F)	`CCCGTCAACACTTGCTGGTTTCC`
Q-miR-199a (F)	`ACACTCCAGCTGGGCCCAGTGTTCAGACT`
UR	`CAGTGCGTGTCGTGGAGT`
U6-F	`CTCGCTTCGGCAGCACA`
U6-R	`AACGCTTCACGAATTTGCGT`
ORF7-F	`CCCTAGTGAGCGGCAATTGT`
ORF7-R	`TCCAGCGCCCTGATTGAA`
GAPDH-F	`CCTTCCGTGTCCCTACTGCCAAC`
GAPDH-R	`GACGCCTGCTTCACCACCTTCT`