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. 2018 Jun 18;14(6):e1007432. doi: 10.1371/journal.pgen.1007432

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© 2018 Ros et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Dissociated mouse commissural neurons were stained for different combinations of DCC and Robo3, and SNARE proteins (Syntaxin1, VAMP2, SNAP25 and Ti-VAMP). Analyses of confocal images from growth cones revealed partial colocalization between: (A) DCC and Syntaxin1; (B) DCC and VAMP2; (C) DCC and SNAP25; and (D) DCC and Ti-VAMP. Colocalization was also found between (E) Robo3 and Syntaxin1; (F) Robo3 and VAMP2; (G) Robo3 and SNAP25; and (H) Robo3 and Ti-VAMP. Arrowheads point to areas of colocalization. Scale bar: 5 μm.