Figure 6.

Silencing ONECUT‐2 (OC‐2) inhibits the Akt/Erk signaling pathway and epithelial–mesenchymal transition (EMT) in ovarian cancer cells. A, Effect on the Akt/Erk signaling pathway after OC‐2 silencing in COV‐504 and SKOV3 cells. (a) Total protein was extracted to assess expression levels of Akt/Erk on COV‐504 and SKOV3 cells transfected with OC2‐siRNAs by Western blot. (b‐e) Erk and Akt phosphorylation (p‐) levels in SKOV3 (b,d) and COV‐504 (c,e) cells were quantified by grayscale analysis. B, EMT‐related markers were detected after OC‐2 silencing by Western blot analysis. (a) N‐cadherin and E‐cadherin expression levels in COV‐504 and SKOV3 cells transfected with OC2‐siRNAs for 48 h. (b‐e) N‐cadherin (b,c) and E‐cadherin (d,e) expression levels in COV‐504 and SKOV3 cells were quantified by grayscale analysis. (f) mRNA expression of Snai1, Snai2, transforming growth factor‐β1 (TGF‐β1), and Twist1 in COV‐504 and SKOV3 cells (transfected with OC2‐siRNA#1) were detected by real‐time PCR. Data were represented as the means ± SD. *P < .05, **P < .01. ACTB, β‐actin; NC, scrambled siRNA