Figure 7.

ONECUT‐2 (OC‐2) silencing inhibits tumor growth and angiogenesis in an ovarian cancer xenograft nude mice model. A, COV‐504 ovarian cancer cells transfected with siRNA#1/scrambled siRNA (siRNA NC) were injected into the right shoulder of BALB/c nude mice (n = 10). Mice were euthanized and the tumors were excised after 21 d. B, Growth curves of tumors. C, Quantitative analysis of tumor weight. D, Paraffin sections of tumors were stained with OC‐2, CD31, VEGFA, and HIF‐1α antibody. The positive rates were statistically analyzed. E, Tumors were stained with CD31 antibody. Microvessel densities were quantified. The numbers of blood vessels at five high‐power fields (×200) per section were counted. F, Positive expression rates of vascular endothelial growth factor A (VEGFA), OC‐2, and hypoxia‐inducible factor‐1α (HIF‐1α) in tumors. Data were represented as the means ± SD. *P < .05, **P < .01