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. 2018 Jun 13;109(7):2221–2234. doi: 10.1111/cas.13633

Table 3.

Primer sequences of real‐time PCR

Gene Forward primer (5′‐3′) Reverse primer (5′‐3′)
VEGFC F: 5′‐GTGTCCAGTGTAGATGAA‐3′
R: 5′‐CCTGTTCTCTGTTATGTTG‐3′
Snai1 F: 5′‐TTTACCTTCCAGCAGCCCTA‐3′
R: 5′‐GACAGAGTCCCAGATGAGCA‐3′
Snai2 F: 5′‐GACACATTAGAACTCACACGG‐3′
R: 5′‐TACACAGCAGCCAGATTCCT‐3′
HGF F: 5′‐ACGCTACGAAGTCTGTGA‐3′
R: 5′‐AAGAATTTGTGCCGGTGT‐3′
HIF‐1α F: 5′‐TCTGGGTTGAAACTCAAGCAACTG‐3′
R: 5′‐CAACCGGTTTAAGGACACATTCTG‐3′
TGF‐β1 F: 5′‐ATGAACTCATTCAGTCACCATAGC‐3′
R: 5′‐CTATCCCCCACTAAAGCAGG‐3′
FGF2 F: 5′‐CCAGTTCGATTCAGTGCCACA‐3′
R: 5′‐GTGTGCTAACCGTTACCTGGCTATG‐3′
Twist1 F: 5′‐AGCTGAGCAAGATTCAGACCCTCA‐3′
R: 5′‐CTGCAGCTTGCCATCTTGGAGT‐3′
GAPDH F: 5′‐TTCACCACCATGGAGAAGGC‐3′
R: 5′‐GGCATGGACTGTGGTCATGA‐3′

F, forward; R, reverse.