Figure 6.

ARNAX + tumor‐associated antigen (TAA) induces memory CD8⁺ T cells that cause rejection of reimplanted tumor cells. A, Scheme of each treatment for PD‐L1^lo EG7‐bearing mice is shown. PD‐L1^lo EG7 were reimplanted into mice in which complete tumor regression was induced by 10 μg ARNAX‐140 + ovalbumin (OVA) treatment. B, Tumor sizes of individual mice were evaluated in the EG7 reimplantation group (green line) and EG7 first‐time implantation group (blue line). C, Mice were killed on day 40. Proportions of OVA‐specific cells, T_EFF (CD44⁺CD62L^loCD127⁻), naive T cells (CD44⁻CD62L^hi), T_CM (CD44⁺CD62L^hi) and T_EM (CD44⁺CD62L^loCD127⁺) among splenic CD8⁺ T cells were analyzed by flow cytometry; n = 3‐5 per group. Student's t test was carried out to analyze statistical significance; ***P < .001