Figure 2.

HSPC159 promoted breast cancer cell proliferation in vitro and in vivo. A, Western blot analysis showed siRNA targeting HSPC159 reduced HSPC159 expression level in MDA‐MB‐231 cells. B, Western blot analysis showed that the protein level of HSPC159 was increased after transfection with pcDNA3.1‐HSPC159 plasmid compared with negative control in MCF‐7 cells. C,D, Cell proliferation rates were measured using CCK‐8 assays in MDA‐MB‐231 cells (C) and MCF‐7 cells (D) after transfection. E,F, Results of EdU assays also confirmed the effect of HSPC159 on cell proliferation. G, Western blot analysis showed shRNA targeting HSPC159 reduced HSPC159 expression level in MDA‐MB‐231 cells. H, Cell proliferation rate was measured using CCK‐8 assays in MDA‐MB‐231 cells after shRNA transfection. I, Knockdown of HSPC159 in MDA‐MB‐231 cells suppressed tumorigenesis in vivo. Tumor volumes were measured on the indicated days. J, Immunohistochemistry for Ki‐67 detection showed that tumor cells in the negative control group showed a higher positivity rate than those in the shRNA‐HSPC159 group (magnification ×200). *P < .05