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. 2018 Jul 3;7:e33910. doi: 10.7554/eLife.33910

Figure 2. DLK is upstream of injury-associated genesimplicated in the establishment of neuropathic pain: Atf3, Gal, Npy, and Serpina3n.

Representative images and quantification of expression of 4 genes whose expression changes following sciatic nerve axotomy: Atf3 (A–C), Gal (D–F), Npy (G–I), and Serpina3n (J–L) by in situ hybridization in DLK WT vs DLK cKO ipsilateral L3 DRG 1 week after SNI (7 dpi). Scale bar 100 µm. *p<0.05, **p<0.01, ***p<0.001 by 2-tailed Student’s t test. Averaged data for n = 4–6 sections quantified in n = 3 male mice per genotype.

Figure 2—source data 1. data plotted in Figure 2; quantification of in situ hybridization for Atf3, Gal, Npy and Serpina3n in DLK WT vs cKO in ipsilateral L4 DRG 7 days post SNI.
elife-33910-fig2-data1.xlsx (37.3KB, xlsx)
DOI: 10.7554/eLife.33910.016
Figure 2—source data 2. data plotted in Figure 2—figure supplement 1; quantification of in situ hybridization for Cckbr, Ecel1, Gpr151, Nts, Sox11, and Sprr1a in DLK WT vs cKO in ipsilateral L4 DRG 7 days post SNI.
elife-33910-fig2-data2.xlsx (38.3KB, xlsx)
DOI: 10.7554/eLife.33910.017

Figure 2.

Figure 2—figure supplement 1. DLK is upstream of multiple genes implicated in the establishment of neuropathic pain.

Figure 2—figure supplement 1.

(A–R) Representative images and quantification of expression of 6 genes whose expression changes following sciatic nerve axotomy: Cckbr (A–C), Ecel1 (D–F), Gpr151 (G–I), Nts (J–L), Sox11 (M–O), Sprr1a (P–R) by in situ hybridization in DLK WT vs DLK cKO L3 DRG 1 week after SNI. Scale bar 100 µm. *p<0.05 and **p<0.01 by 2-tailed Student’s t test. Averaged data for n = 4–6 sections quantified for n = 3 male mice per genotype.