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. 2018 Jul 3;7:e36354. doi: 10.7554/eLife.36354

Figure 7. Selective LOXL2/LOXL3 inhibition modifies collagen fibril assembly.

Figure 7.

IPF fibroblasts were grown for 6 weeks in the 3D in vitro model of fibrosis with vehicle control, concentrations of PXS-S2A or 1 mM BAPN, as indicated. (A) Representative images of histological sections stained with picrosirius red and viewed under plane polarised light, scale bar: 50 μm. (B) Representative transmission electron microscopy images of collagen fibrils in transverse (upper panel, scale bar 200 nm) and longitudinal (lower panel, scale bar 100 nm) cross-section. Images are representative of the mean fibril diameter. (C) Collagen fibril diameter measured in transverse section (300 fibrils per treatment measured by a blinded observer from one experiment). Data are representative of measurements from two independent experiments. Bars show the median value. ****p<0.0001 by non-parametric one-way ANOVA (Kruskal-Wallis test) with Dunn’s multiple comparison test. (D) Collagen solubility of whole cultures assessed by sequential extractions using TBS, 0.5M acetic acid, and pepsin. Each fraction and the final insoluble residue were hydrolysed and assayed for hydroxyproline. Bars are mean +range of two IPF donors.