Table 1.
KD for Homodimerization of Type II Cadherin Wild-Type, Chimera, and Mutant Protein Fragments Determined by Analytical Ultracentrifugation. See also Table S2.
| Cadherin | KD (mM) | Description |
|---|---|---|
| Cadherin-6 | 3.1 ± 0.1a,b | wild-type |
| Cadherin-9 | 17.0 ± 1.1c | wild-type |
| Cadherin-10 | 42.2 ± 2.7c | wild-type |
| Cadherin-8 | 15.0 ± 0.4c | wild-type |
| Cadherin-11 | 33.8 ± 0.2c | wild-type |
| Cadherin-24 | 8.2 ± 0.3 | wild-type |
| Cadherin-7 | 32.2 ± 0.8 | wild-type |
| Cadherin-12 | 8.3 ± 1.6 | wild-type |
| Cadherin-18 | 16.8 ± 0.2 | wild-type |
| Cadherin-20 | 9.3 ± 0.6 | wild-type |
| Cadherin-22 | 3.9 ± 0.2 | wild-type |
| cad-6EC111EC2 | 5.6 ± 0.2 | chimera cad-6EC111EC2 |
| cad-11EC16EC2 | 15.6 ± 0.9 | chimera cad-11EC16EC2 |
| Cadherin-6 Y20L H97Q (LQ) | 9.63 ± 1.3 | specificity mutant |
| Cadherin-6 M3V Y20L H97Q E89P (VLQP) | 6.73 ± 0.8 | specificity mutant |
| Cadherin-11 L20Y Q97H (YH) | 11.1 ± 2.1 | specificity mutant |
| Cadherin-11 V3M L20Y Q97H P89E (MYHE) | 8.2 ± 0.8 | specificity mutant |
a
Errors are the SD from two or more experiments.
b
Previously reported in Harrison et al. (2010).
c
Previously reported in Brasch et al. (2011).