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. Author manuscript; available in PMC: 2018 Aug 28.
Published in final edited form as: Chem Rev. 2018 Jan 31;118(4):1917–1950. doi: 10.1021/acs.chemrev.7b00534

Figure 13. Micro-nuclear magnetic resonance.

Figure 13

(a) Assay schematics to maximize magnetic nanoparticle (NMP) binding onto EVs. A two-step bio-orthogonal click chemistry was used to label EVs with MNPs. (b) The microfluidic system for on-chip detection of circulating EVs is designed to detect MNP-targeted vesicles, concentrate MNP-tagged vesicles (while removing unbound MNPs) and provide in-line NMR detection. (c) GBM markers (EGFR, EGFRvIII, PDGFR, PDPN, EphA2 and IDH1 R132H), a positive EV control marker (HSP90), as well as host cell markers (CD41, MHCII) were profiled in both parental cells (left) and their corresponding EVs (right). Using a four-GBM marker combination (EGFR, EGFRvIII, PDPN and IDH1 R132H), GBM derived EVs could be distinguished from host cell–derived EVs. MFI, mean fluorescence intensity; HBMVEC, human brain microvascular endothelial cell; NHA, normal human astrocyte; buffy coat and plasma were isolated from whole blood donated by healthy volunteers. Reprinted with permission from Ref 20. Copyright 2012 Nature Publishing Group.