Figure 22. Localized surface plasmonic resonance for detecting EV RNA.
(a) Scanning electron microscopy (SEM) image of gold nanoprisms for LSPR-based sensors fabrication. (b) Assay schematic of the LSPR assay. The immobilized nanoprisms are functionalized with capturing DNA probes and polyethylene glycol spacers. Upon hybridization of the miRNA target (miR-10b) with the capturing DNA probes, the LSPR resonant peak shifts. (c) miR-10b detection in clinical samples from pancreatic ductal adenocarcinoma (PDAC; left) or chronic pancreatitis (CP; right) patients. Determination of the miRNA levels was performed in plasma, exosomes and supernatants, respectively. Reprinted with permission from Ref 192. Copyright 2015 American Chemical Society.