Table 2.
Comparison of high-throughput bulk EV preparation methods.
| Platform | Mechanism of enrichment |
Advantages | Limitations |
|---|---|---|---|
| Ultracentrifugation | Density |
|
|
| Sucrose-gradient centrifugation | Density |
|
|
| Co-precipitation | Surface charge |
|
|
| Size-exclusion chromatography | Size and molecular weight |
|
|
| Field flow fractionation | Size and molecular weight |
|
|