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. 2018 Jul 3;8:9987. doi: 10.1038/s41598-018-28363-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Filipin, but not amphotericin B, inhibits release of PS-exposing EVs. (a,b) Washed platelets were treated with the indicated concentrations of filipin or amphotericin B, or vehicle as control prior to stimulation with A23187 (10 µM). CD41⁺/annexin V⁺ EVs were identified as in Fig. 1. (a). EVs release in (a) are expressed as % of vehicle-treated, A23187-stimulated platelets (n = 5; **p < 0.01). (b) Shows the percentage of annexin V-positive platelets from these experiments.