Fig. 2. Generation of cone-conditional PKM2-KO mice.

Schematic diagram of loxP floxed PKM2 loci. Cone photoreceptor-specific PKM2 KO mice were generated by breeding mice with an exon 10 floxed PKM gene with mice that express Cre recombinase under the control of human red/green pigment promoter (a). Primer pairs P1 and P2 were used to identify the wild-type, cone-Cre, and the floxed PKM2 alleles (b, c). Cryosections were prepared from PKM2-WT and cone-cre-PKM2-KO mouse retinas and were immunostained with an anti-Cre antibody (d, e). Prefer-fixed sections of 8-week-old PKM2-WT (g) and rod-cre PKM2-KO (h) mouse retinas were subjected to immunofluorescence with anti-rhodopsin (red) and PKM1 (green) antibodies. Prefer-fixed sections of 12-week-old PKM2-WT (j, k) and cone-cre PKM2-KO (l, m) mouse retains were stained with PNA (red) and PKM1 (green) antibodies, and images were captured on both dorsal and ventral regions of the retina. Panels (f) and (i) represent the omission of primiary antibody. ROS rod outer segments, RIS rod inner segments, ONL outer nuclear layer, OPL outer plexiform layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer. Scale bar = 50 μm