Fig. 5. The function and structure of the 28-week-old cone-cre PKM2-KO mouse retina.

Scotopic a-wave (a), scotopic b-wave (b), photopic b-wave (c), and cone flicker (d) electroretinographic analysis was performed on 28-week-old PKM2-WT and cone-cre PKM2-KO mice. Scotopic a-wave and scotopic b-wave amplitudes were carried out at different flash intensities (−3.4, −2.4, −1.4, −0.4, 0.6, 1.6, and 2.6 log cd s/m²), whereas photopic b-wave amplitudes were performed at a flash intensity of 3.3 log cd s/m². Isolated cone photoreceptor components of the photopic flicker ERG were done at 3, 10, 20, and 30 Hz. Data are mean ± SEM (n = 13). Significance, if any, is indicated on each panel. Panels (e–l) represent the difference of expression of S-opsin (S-opsin positive cones) and M-opsin (M-opsin positive cones) in dorsal and ventral regions of the retina from 28-week-old PKM2-WT and cone-cre PKM2-KO mice. Panel (m) represents the quantification of the number of cones in dorsal and ventral regions of the retina counted starting from the optic nerve head (ONH). Data are mean ± SEM (n = 8). Significance, if any, is indicated on each panel. Scale bar = 50 μm. The images shown are representative of six retinas examined from PKM2-WT and cone-cre PKM2-KO mice