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. 2018 Jul 3;9(7):736. doi: 10.1038/s41419-018-0777-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Architecture of the miR34a locus (hg38, RefSeq) including miR34a HG, mature miR34a, and lncTAM34a (LINC01759). H3K4me3 ChIP-seq data, indicating the active promoter region, and conservation are also shown. b Semiquantitative PCR data from the screening of a panel of cancer cell lines. Wild-type TP53 is indicated with +, − indicates null, and +* represents either a nonnull TP53 mutation or wild-type TP53 with mechanisms present that inhibit its function (e.g., SV40 large T antigen in HEK293T cells). c TCGA correlation analysis. Expression was log2 normalized to the maximum expression value. Nonsynonymous TP53 mutations are indicated on the top of the plot (cancer type abbreviation definitions and corresponding statistics are in Fig. 1-Supplement 1). d 3′-RACE sequencing results and the annotated lncTAM34a (LINC01759) are shown. e Semiquantitative PCR results from the primer walk assay (i.e., common reverse primer (exon 2) and forward primers (F10–F15) staggered upstream of lncTAM34a’s annotated start site) performed using HEK293T cells (Fig. 1-Supplement 2a details primer placement). f Coding-potential analysis assessed via the coding-potential assessment tool including lncTAM34a, two known noncoding RNAs (HOTAIR and XIST), and three protein-coding RNAs (β-actin, Tubulin, and MYC)