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. 2018 Jul 3;8:9993. doi: 10.1038/s41598-018-28404-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of tree shrew collagen-induced arthritis (TsCIA). (a) Time schedule for the establishment of TsCIA. (b) Clinical scores of the control and TsCIA groups (n = 20 tree shrews per group). AU indicates arbitrary unit. (c) Representative X-ray images from control and TsCIA forelimbs at day 28. R, right and L, left. Arrows indicate damaged joints in TsCIA. (d–i) Histochemical analysis of joint cross-sections from the same tree shrews shown at day 21 and day 45. H&E staining shows the endogenous chondrocytes in (d–f). Sirius red and solid green staining show cartilage damage (arrows) in (g–i). Scale bar, 200 μm.