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. 2018 Jul 3;8:9993. doi: 10.1038/s41598-018-28404-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Up-regulation of CXCL10 in the lungs of TsCIA. (a–d) mRNA expression and distribution of CXCL10 were detected by in situ hybridization (ISH) in TsCIA lungs at day 45. Representative ISH signals are indicated by arrows. Hybridizations were performed by antisense (a and b) or sense probes (c and d), respectively. Control (a and c); TsCIA (b and d); Scale bar, 200 μm. (e) ISH signal area was analyzed by NIH ImageJ software (*p < 0.05. Error bars indicate SEM). (f) Quantification of mRNA levels of chemokines in control lungs and TsCIA lungs (n = 4 tree shrews per group, **p < 0.01, ***p < 0.001. Error bars indicate SEM).