Figure 2.

CAP induces Ca2⁺ primarily from intracellular sources. (A,B) Cytoplasmic Ca²⁺ levels stimulated by 30 s CAP exposure in the presence or absence of extracellular Ca²⁺. Mel Im ((A), n = 660 and 464) and Mel Juso. ((B), n = 492 and 397) were washed for 5 min in pbECS before CAP in a solution with or without Ca²⁺. (C,D) Smoothed first derivative (dR/dt) of experiment without extracellular Ca²⁺ (grey trace). The effect due to the presence of extracellular Ca²⁺ is calculated from the difference of experiment with and without extracellular Ca²⁺ (black trace). Data are shown as mean and 99% confidence interval.